(Untitled)

[sewnupheart]

• aspirate media
  
• [5 mL] sterile PBS
  
• aspirate PBS
  
• [3 mL] trypsin/EDTA
  
• incubate [37 C/3 minutes]
  
• tap to remove cells from flask
  
• [3 mL] culturing media
  
• break cell clumps and pipette into [15 mL] centrifuge tube
  
• incubate [3 minutes/700 rpm/26 C]
  
• aspirate supernatant
  
• resuspend in [3 mL] culturing media
  
• put [1/10] of final volume into each [T-75] flask
  

Comments

join

[drrosensteinmd]

Re: join

[sewnupheart]

sure, i'll take a look.

just humor me - how did you find me? lol.

Re: join

[drrosensteinmd]

i searched rating interests and went to a rating community then randomly picked your name =)

thanks for checkin it out.

[__thejealous]

yo its lisa, add this journal!

[_all_for_you_]

[_all_for_you_]

hey andrew, i think it's in your best interests to find me. I could show you a really good time. see ya soon sexy

[sewnupheart]

i think it's in your best interest to identify yourself or fuck off.

[_all_for_you_]

Andrew?