Jun 18, 2004 15:09
- aspirate media
- [5 mL] sterile PBS
- aspirate PBS
- [3 mL] trypsin/EDTA
- incubate [37 C/3 minutes]
- tap to remove cells from flask
- [3 mL] culturing media
- break cell clumps and pipette into [15 mL] centrifuge tube
- incubate [3 minutes/700 rpm/26 C]
- aspirate supernatant
- resuspend in [3 mL] culturing media
- put [1/10] of final volume into each [T-75] flask
-
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sorry dood. .x.
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