(Untitled)

[sewnupheart]

• aspirate media
  
• [5 mL] sterile PBS
  
• aspirate PBS
  
• [3 mL] trypsin/EDTA
  
• incubate [37 C/3 minutes]
  
• tap to remove cells from flask
  
• [3 mL] culturing media
  
• break cell clumps and pipette into [15 mL] centrifuge tube
  
• incubate [3 minutes/700 rpm/26 C]
  
• aspirate supernatant
  
• resuspend in [3 mL] culturing media
  
• put [1/10] of final volume into each [T-75] flask
  

Comments

[sewnupheart]

aww, thanks!

[signsnatcher84]

i think the bare feet complete the outfit well <3

[xsammox]

hahahhahahahahha omg yes i was just thinking that!!!!

:] <3

[sewnupheart]

hehe. <3.

[sewnupheart]

the only thing preppier would be a pair of birkenstocks and a scholarship to yale. heh.

<3.

[___promiseme]

you said i was gorgeous in a community.
add me<3

[sewnupheart]

hawtsex, wasn't it?

sure thing, hon.
<3.

[___promiseme]

it sure was.
: )

[nothingworse]

This entry makes you seem very doctor-like. And I agree with the others, preppy works, aha.

[anonymous]

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