Comments | lab_gripes: Reverse Transcription PCR

ase in lab_gripes

Reverse Transcription PCR

Jul 26, 2011 23:51

Why does my RT-PCR suck? Because the RT-extension primer has a nasty hairpin Tm one degree below the protocol's RNA-to-DNA temp. Why didn't I know this? I assumed the previous worker checked the primer design before placing the order. Why didn't using the alternate primers help? Because they were designed by a second person who hadn't checked their ( Read more... )

Comments 1

The First Rule Of Lab Existence broken010408 July 28 2011, 01:49:38 UTC

" if it isn't written down, signed, dated, and reviewed in a timely manner It Didn't Happen" according to my first and beloved supervisor J (repeatedly- guess its a FDA thing )
biotech drove her back to teaching btw
biotech drove most of us to other careers in fact- lots of nurses, teachers,CNAs...