Tum te tum
Have been sitting next to the Mass-Spec all day and now my hearing in one ear is all funky. Curse you, Mass Spec.
However! Experiments are working! I really ought to talk to Katherine about how cool it is that it's finally working, because then I won't have to explain every part of it--since I've already yayed at my coworkers. But suffice it to say, the project I've been failing on for 4 months now is FINALLY responding, and now I've got super-cool COMMD1-GFP constructs and we're gonna make our mammalian cells fluoresce the fuck up. Dude.
Furthermore, protein expression from Sf9 cells worked--it's just not coming out in the right place. Rather than eluting from the column, it's just not binding. Why? No clue. Next I'll do a Western with an anti-his tag antibody, to see if my his tag is still on my protein or if it has fallen off (tears). Is this interesting to anyone?
Today I borrowed Peter's bike so that I can bike home from the climbing gym. Fuckin' bus. The super nice thing about a bike is that you don't rely on any kind of schedule--you just leave when you want and get home when you get home. No standing aimlessly in the cold, waiting for some driver to notice you in the dark. No creepy dudes at the bus stop. Bikedy bike. I really do need my own.
But that comes after tickets to Minnesota to see Anna and Emily sing their pants off (hopefully not, that might be embarrassing) and also having my bunny stripped of a uterus. It sounds so nice when you put it that way, doesn't it?
Also! I really need to clean up my room and get more lamps so it's not dark and cluttered and depressing. Indeed. It's still not completely clean from when I moved in. I might actually want to spend time there if it's clean and well-lit.
So yeah.
However! Experiments are working! I really ought to talk to Katherine about how cool it is that it's finally working, because then I won't have to explain every part of it--since I've already yayed at my coworkers. But suffice it to say, the project I've been failing on for 4 months now is FINALLY responding, and now I've got super-cool COMMD1-GFP constructs and we're gonna make our mammalian cells fluoresce the fuck up. Dude.
Furthermore, protein expression from Sf9 cells worked--it's just not coming out in the right place. Rather than eluting from the column, it's just not binding. Why? No clue. Next I'll do a Western with an anti-his tag antibody, to see if my his tag is still on my protein or if it has fallen off (tears). Is this interesting to anyone?
Today I borrowed Peter's bike so that I can bike home from the climbing gym. Fuckin' bus. The super nice thing about a bike is that you don't rely on any kind of schedule--you just leave when you want and get home when you get home. No standing aimlessly in the cold, waiting for some driver to notice you in the dark. No creepy dudes at the bus stop. Bikedy bike. I really do need my own.
But that comes after tickets to Minnesota to see Anna and Emily sing their pants off (hopefully not, that might be embarrassing) and also having my bunny stripped of a uterus. It sounds so nice when you put it that way, doesn't it?
Also! I really need to clean up my room and get more lamps so it's not dark and cluttered and depressing. Indeed. It's still not completely clean from when I moved in. I might actually want to spend time there if it's clean and well-lit.
So yeah.