Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen/ Пауки Вызывают Астму
Int Arch Allergy Immunol. 2010 Dec 23;155(2):180-186. [Epub ahead of print]
Arginine Kinase from the Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen.
Bobolea I, Barranco P, Pastor-Vargas C, Iraola V, Vivanco F, Quirce S.
Source
Allergy Department, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain.
Spider Holocnemus pluchei (Daddy Long Legs or Marbled Cellar spider)
Image borrowed from http://dermatology.cdlib.org/DOJvol5num2/special/recluse.html
Вкратце содержание статьи: Кожные пробы, проведенные с вытяжкой из паука Holocnemus pluchei (Косиножка по-русски?), у 31-летнего мужчины с астматическими симптомами, проявившимися после вдыхания пыли в помещении с наличием большого количества вышеуказанных пауков, выявили аллергическую сенсибилизацию связанную с бронхиальными симптомами.
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Abstract
Background: We report a 31-year-old farmer whose work consists in handling cereal and vegetables, who consulted our clinic because of asthma symptoms after inhalation of dust during manipulation of the deposited material, usually inside the warehouse. Methods and Results: Skin prick tests and specific immunoglobulin E (IgE) determinations were negative with common aeroallergens. The patient noted the presence of many spiders in the warehouse, which were identified as the cellar spider Holocnemus pluchei and the common house spider, Tegenaria domestica. Extracts of spider bodies brought in by the patient were obtained and used to perform in vivo and in vitro studies. Molecular characterization of IgE-binding bands was performed by mass spectrometry. We obtained positive prick tests to the extracts of the bodies of both spiders. Immunoblotting displayed different bands in both spider extracts, in a range of 20-70 kDa. All were hemocyanins, except for a 17-kDa protein of Holocnemus identified as an arginine kinase (AK). Bronchial challenge was positive with the extract of the cellar spider and with the AK, but was negative with the domestic house spider. Conclusion: We present the first case of respiratory allergy due to sensitization to AK from a common spider, confirmed by bronchial provocation tests.
Copyright © 2010 S. Karger AG, Basel.
PMID: 21196763 [PubMed - as supplied by publisher]
Male House spider, Tegenaria domestica, close-up of body.
Male House spider, Tegenaria domestica, head-on view.
Female House spider, Tegenaria domestica, head-on view.
Female House spider, Tegenaria domestica, side view.
Photos are borrowed from http://www.eurospiders.com/Tegenaria_domestica.htm
There are more photos there.
Other -common names- for the spider Holocnemus pluchei are Daddy Long Legs & Marbled Cellar spider.
More photos of this spider are here http://www. eurospiders.com/Holocnemus pluchei.htm
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Arginine Kinase from the Cellar Spider (Holocnemus pluchei): A New Asthma-Causing Allergen.
Bobolea I, Barranco P, Pastor-Vargas C, Iraola V, Vivanco F, Quirce S.
Source
Allergy Department, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain.
Spider Holocnemus pluchei (Daddy Long Legs or Marbled Cellar spider)
Image borrowed from http://dermatology.cdlib.org/DOJvol5num2/special/recluse.html
Вкратце содержание статьи: Кожные пробы, проведенные с вытяжкой из паука Holocnemus pluchei (Косиножка по-русски?), у 31-летнего мужчины с астматическими симптомами, проявившимися после вдыхания пыли в помещении с наличием большого количества вышеуказанных пауков, выявили аллергическую сенсибилизацию связанную с бронхиальными симптомами.
.
Abstract
Background: We report a 31-year-old farmer whose work consists in handling cereal and vegetables, who consulted our clinic because of asthma symptoms after inhalation of dust during manipulation of the deposited material, usually inside the warehouse. Methods and Results: Skin prick tests and specific immunoglobulin E (IgE) determinations were negative with common aeroallergens. The patient noted the presence of many spiders in the warehouse, which were identified as the cellar spider Holocnemus pluchei and the common house spider, Tegenaria domestica. Extracts of spider bodies brought in by the patient were obtained and used to perform in vivo and in vitro studies. Molecular characterization of IgE-binding bands was performed by mass spectrometry. We obtained positive prick tests to the extracts of the bodies of both spiders. Immunoblotting displayed different bands in both spider extracts, in a range of 20-70 kDa. All were hemocyanins, except for a 17-kDa protein of Holocnemus identified as an arginine kinase (AK). Bronchial challenge was positive with the extract of the cellar spider and with the AK, but was negative with the domestic house spider. Conclusion: We present the first case of respiratory allergy due to sensitization to AK from a common spider, confirmed by bronchial provocation tests.
Copyright © 2010 S. Karger AG, Basel.
PMID: 21196763 [PubMed - as supplied by publisher]
Male House spider, Tegenaria domestica, close-up of body.
Male House spider, Tegenaria domestica, head-on view.
Female House spider, Tegenaria domestica, head-on view.
Female House spider, Tegenaria domestica, side view.
Photos are borrowed from http://www.eurospiders.com/Tegenaria_domestica.htm
There are more photos there.
Other -common names- for the spider Holocnemus pluchei are Daddy Long Legs & Marbled Cellar spider.
More photos of this spider are here http://www. eurospiders.com/Holocnemus pluchei.htm
.