Published methods for inducing the rapid autolytic breakdown of yeast RNA include heat shok , starvation, 3 0 and chilling to 0°C.32W e have found that exposure of a suspension of commercial baker’s yeast to a temperature of 70°C for 1 min, a time considerably exceeding that recommended for Candida yeast, causes degradation of RNA to commence without delay when the suspension is subsequently incubated at 50°C.
Trevelyan, W. E. (1976). Autolytic methods for the reduction of the purine content of baker's yeast, a form of single‐cell protein. Journal of the Science of Food and Agriculture, 27(8), 753-762.
Ohta, S., Maul, S., Sinskey, A. J., & Tannenbaum, S. R. (1971). Characterization of a heat-shock process for reduction of the nucleic acid content of Candida utilis. Applied microbiology, 22(3), 415-421.
Comments 8
- Почему это физики всегда требуют такое дорогое оборудование? Вот, например, математики просят лишь деньги на бумагу, карандаши и ластики. Подумав, добавил, а философы, те ещё лучше, им даже ластики не нужны.
а эти и карандаши с бумагой сами купят!
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http://www.liveinternet.ru/users/bahera/post395331018/
https://mariana-aga.livejournal.com/tag/%D0%B4%D1%80%D0%BE%D0%B6%D0%B6%D0%B8
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http://elibrary.nuft.edu.ua/library/TopicDescription?topic_id=20108&page=1
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Oriental Yeast Co. (Tokyo, Japan),
Toyo Jozo Co. (Tokyo, Japan),
Kyowa Hakko Kogyo Co. (Tokyo, Japan),
Kaneka Co. (Osaka, Japan),
Asahi Chemical Industry Co. (Tokyo, Japan)
Hirasawa, R., & Yokoigawa, K. (2001). Leavening ability of baker's yeast exposed to hyperosmotic media. FEMS Microbiology letters, 194(2), 159-162.
см. https://t-kudelina.livejournal.com/123733.html?view=132693#t132693
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shok , starvation, 3 0 and chilling to 0°C.32W e have found that exposure of a suspension of
commercial baker’s yeast to a temperature of 70°C for 1 min, a time considerably exceeding that
recommended for Candida yeast, causes degradation of RNA to commence without delay when
the suspension is subsequently incubated at 50°C.
Trevelyan, W. E. (1976). Autolytic methods for the reduction of the purine content of baker's yeast, a form of single‐cell protein. Journal of the Science of Food and Agriculture, 27(8), 753-762.
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https://mariana-aga.livejournal.com/245473.html?thread=11278305#t11278305
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