(no subject)
Holy crap. It feels so good to be a fangirl again.
So, the phrase tossed around is, "You never forget your first Doctor."
I get it. Now I get it. Now that Matt Smith is going to be replaced, I realize I've fallen in love with him. Don't leave us, Matt Smith! It won't be the same! *sob!* It's this bizarre profound sense of loss....
It just won't be the same.
Stuff You Should Know did a podcast on asexuality, and mentioned the Doctor as one of the popular asexual characters. I guess that turned out not to be true, which is a pity. I rather prefer him to be an untouchable godlike creature devoted to Everyone rather than to River.
In less important news, I'm seeing a light at the end of the tunnel, and it might be a train.
I'm looking for a job for fall of next year, because my funding runs out. But we don't have our knockdowns, and my transcription factor is being Uncooperative and turning on anti-inflammatory genes instead of inflammatory ones. There are about 500 different ways it could be doing this. I'm starting to suspect it's more of a negative feedback mechanism, but my boss is clinging to the pro-inflammatory idea. So... we continue to flounder around in the dark. The stupid BATF family of proteins interacts with AP-1 factors, turns on some genes, represses others by sequestering said AP-1 factors, ignores some AP-1 members entirely, interacts with chromatin remodeling machinery, and basically is screwing with me. I'd better figure out where to focus, fast.
Also, out of 8x96 cells isolated by sorting, 6 proliferated, and 3 proliferated enough to put in several wells, and now two of those 3 are suddenly blebbing out on me. And my other cells are randomly struggling. Wtc. I don't even know what to suspect. Media? And also, bacteria not growing properly. WTC. Why is it always the stupid simple things that go wrong in research? Oh sure, I can mutate a plasmid in two places and stick it in bacteria and get it out and then go through the whole complicated cloning procedure without a hitch, but I can't get my normal cells or even bacteria to grow. ALKSDJFWEBLEARG.
Finally, I don't know how like moms and stuff do it. I'm so burned out by the end of the week, I find myself complaining and unwilling to devote all my attention to cleaning. I feel indignant because I've had no fun all week. Oh boo hoo. Starving kids in Africa. at least they have time to play I am a terrible person.
...I'm going to go back to mourning Matt Smith, and watching Doc Martin while doing yoga. Shoving the laundry in the bedroom can happen 30 minutes before Bible Study tomorrow.
So, the phrase tossed around is, "You never forget your first Doctor."
I get it. Now I get it. Now that Matt Smith is going to be replaced, I realize I've fallen in love with him. Don't leave us, Matt Smith! It won't be the same! *sob!* It's this bizarre profound sense of loss....
It just won't be the same.
Stuff You Should Know did a podcast on asexuality, and mentioned the Doctor as one of the popular asexual characters. I guess that turned out not to be true, which is a pity. I rather prefer him to be an untouchable godlike creature devoted to Everyone rather than to River.
In less important news, I'm seeing a light at the end of the tunnel, and it might be a train.
I'm looking for a job for fall of next year, because my funding runs out. But we don't have our knockdowns, and my transcription factor is being Uncooperative and turning on anti-inflammatory genes instead of inflammatory ones. There are about 500 different ways it could be doing this. I'm starting to suspect it's more of a negative feedback mechanism, but my boss is clinging to the pro-inflammatory idea. So... we continue to flounder around in the dark. The stupid BATF family of proteins interacts with AP-1 factors, turns on some genes, represses others by sequestering said AP-1 factors, ignores some AP-1 members entirely, interacts with chromatin remodeling machinery, and basically is screwing with me. I'd better figure out where to focus, fast.
Also, out of 8x96 cells isolated by sorting, 6 proliferated, and 3 proliferated enough to put in several wells, and now two of those 3 are suddenly blebbing out on me. And my other cells are randomly struggling. Wtc. I don't even know what to suspect. Media? And also, bacteria not growing properly. WTC. Why is it always the stupid simple things that go wrong in research? Oh sure, I can mutate a plasmid in two places and stick it in bacteria and get it out and then go through the whole complicated cloning procedure without a hitch, but I can't get my normal cells or even bacteria to grow. ALKSDJFWEBLEARG.
Finally, I don't know how like moms and stuff do it. I'm so burned out by the end of the week, I find myself complaining and unwilling to devote all my attention to cleaning. I feel indignant because I've had no fun all week. Oh boo hoo. Starving kids in Africa. at least they have time to play I am a terrible person.
...I'm going to go back to mourning Matt Smith, and watching Doc Martin while doing yoga. Shoving the laundry in the bedroom can happen 30 minutes before Bible Study tomorrow.