Fun with Molecular Genetics
So far, 335 is much easier than 334, and is going at a really slow pace (hopefully we don't get a ridiculously difficult exam with material that we've never seen). Also, most of it is stuff I've already learned before (but in far less detail), so it's a nice little review.
On Friday, the lecture was on cloning, and the teacher briefly went over the topic of plasmids* as vectors and restriction enzymes. After, he asked us, if we were working in a lab and were told to get clones of of lac+ cells, how would we do it based on what we have just learned? (Ie-how could we get many copies of the lacZ gene?)
I whispered to my friend, "Use a restriction enzyme to cut out the gene from a lac+ cell and insert it into a plasmid." This was the correct answer, but when I raised my hand to answer, I said, "Get a pUC19 plasmid!" Yeah, it was a cheeky answer, but I couldn't resist. My teacher laughed.
*The teacher had also given us examples of plasmids that were regularly used in cloning, such as the pUC19 plasmid (which I had actually used in a cell biology lab), so while my answer was a bit cheeky and not the answer he was looking for, technically it was correct!
On Friday, the lecture was on cloning, and the teacher briefly went over the topic of plasmids* as vectors and restriction enzymes. After, he asked us, if we were working in a lab and were told to get clones of of lac+ cells, how would we do it based on what we have just learned? (Ie-how could we get many copies of the lacZ gene?)
I whispered to my friend, "Use a restriction enzyme to cut out the gene from a lac+ cell and insert it into a plasmid." This was the correct answer, but when I raised my hand to answer, I said, "Get a pUC19 plasmid!" Yeah, it was a cheeky answer, but I couldn't resist. My teacher laughed.
*The teacher had also given us examples of plasmids that were regularly used in cloning, such as the pUC19 plasmid (which I had actually used in a cell biology lab), so while my answer was a bit cheeky and not the answer he was looking for, technically it was correct!