Below the cut so it doesn't take up too much space.
1
Sam Winchester
2
Dean Winchester
3
Mary Winchester
4
Castiel
5
John Winchester
6
Jo Harvelle
7
Bela Talbot
8
Victor Henricksen
9
Ruby
10
Balthazar
11
Jody Mills
12
Meg
13
Jessica Moore
13
Ellen Harvelle
15
Amelia Richardson
16
Lisa Braeden
17
Krissy Chambers
18
Tessa (the reaper)
19
Naomi
20
Death (the horseman)
21
Claire Novak
22
Gadreel(/Ezekiel)
23
The Impala
24
Dick Roman
25
Lilith
26
Abaddon
27
Hannah
27
Anna Milton
29
Sarah Blake
30
Zachariah
31
Raphael
32
Rowena
33
Uriel
34
Kevin Tran
35
Lucifer
36
Kate (the werewolf)
37
Cole Trenton (lol)
38
Charlie Bradbury
39
Rufus Turner
40
Benny Lafitte
41
Linda Tran
42
The Alpha Vampire (from S6)
43
Amelia Novak
44
Gwen Campbell
45
Michael
46
Donna Hanscum
47
Christian Campbell
48
Rachel
49
Metatron
50
Jimmy Novak
51
Frank Deveraux
51
Alistair
51
Samandriel
54
Ben Braeden
54
Azazel
54
Crowley
57
Bobby Singer
57
Garth Fitzgerald III
57
Samuel Campbell
60
Bartholomew
61
Adam Milligan
61
Gabriel
61
Ed Zeddmore & Harry Spengler (Ghostfacers)
61
Chuck Shurley
65
The Styne Family
65
Cain
67
Becky Rosen
67
Gordon Walker
The bottom ones are more inline, than the top ones. Perhaps I'm more clearly defined on who I dislike. I'm surprised that Garth and Adam are as low as they are - I'd pegged them more for middle rankers, and I'm suspicious that Christian Campbell is at 47 - I'm fairly sure I clicked whoever he was against, each time he popped up. However Bobby, Samuel, Becky, Gabriel, yeah not surprised.
The top 15 are relatively accurate, but I'm not sure I'd put Mary as high as that. I do like her a lot (as witnessed by constant clicking) but I'm not sure I like her more than I like Bela, Ruby, Tessa etc. I'm also really surprised by John being in the top 10. I don't subscribe to John hate, but I also don't find him very interesting, and I would never say I like him particularly. Certainly, if he was in my top 15, it'd be towards the bottom. Jo, Bela, Victor, Ruby, Balthazar, Jody, these are all good picks though.
ETA: I've decided that John should be at 25. Below Dick Roman (who I thought would be higher up as villains go) but above Lilith. Mary should be at no 10, and I would move Gwen, Anna, Kevin and Uriel up about 15 places each into my top 20 at least.