feeling contrary about grammar today
la la la
ostensibly 9 days away from when i am supposed to start doing experiments in nyc
there is this idea that i will isolate dna from a strain of bacterial cells - which i just did earlier this afternoon, albeit in underwhelming amounts - and insert it into a different bacterial strain - which does not exist in a suitable form, so i need to streak out a deep-frozen culture out on solid media plates - which i just poured
and then manage to on the very first try successfully insert the dna into the different bacterial strain - which, btw, i need to chemically prepare which i plan to do tomorrow - and then grow it up and successfully overproduce the protein
...which, oh yeah, has to be done such that it incorporates an otherwise toxic amino acid derivative at the last step....
so i can then purify it without any major impurities or degradation projects and then somehow assemble a few samples for experiments of multiple components in carefully determined amounts which i still need to figure out....
oh yes, this is going to be awesome
and by awesome i mean "holy shit the carnage is going to be EPIC!"
oh and in other news i cant stop looking at the tumblr of this girl who used to work down the hall from me in philly mostly since she keeps posting pictures of herself in a bikini
la la la
so in short
total frustration, in more than one way,
total dire fear
complete amusement at how horribly this is going to turn out
and maybe im turning into a republican since i had this awful thought come to mind
you go to research with the proteins you have, not the proteins you wish you had
ok, i should go now
ostensibly 9 days away from when i am supposed to start doing experiments in nyc
there is this idea that i will isolate dna from a strain of bacterial cells - which i just did earlier this afternoon, albeit in underwhelming amounts - and insert it into a different bacterial strain - which does not exist in a suitable form, so i need to streak out a deep-frozen culture out on solid media plates - which i just poured
and then manage to on the very first try successfully insert the dna into the different bacterial strain - which, btw, i need to chemically prepare which i plan to do tomorrow - and then grow it up and successfully overproduce the protein
...which, oh yeah, has to be done such that it incorporates an otherwise toxic amino acid derivative at the last step....
so i can then purify it without any major impurities or degradation projects and then somehow assemble a few samples for experiments of multiple components in carefully determined amounts which i still need to figure out....
oh yes, this is going to be awesome
and by awesome i mean "holy shit the carnage is going to be EPIC!"
oh and in other news i cant stop looking at the tumblr of this girl who used to work down the hall from me in philly mostly since she keeps posting pictures of herself in a bikini
la la la
so in short
total frustration, in more than one way,
total dire fear
complete amusement at how horribly this is going to turn out
and maybe im turning into a republican since i had this awful thought come to mind
you go to research with the proteins you have, not the proteins you wish you had
ok, i should go now