While some games like Half-Life 2 can forgo such gaming convention as bosses and still be good, I still think that well designed bosses are very important to any type of video game were action is involved.
So to waste some time, I compiled my current list of the bosses in video games that I really stuck out to me. There are 20 and I left MANY off the list. Also, this list changes as the years go by so if I do it again there will be most likely new big bad guys in the slots.
1) Super Bowser -- Super Mario 64 (Nintendo 64)
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2) Great Mighty Poo -- Conker's Bad Fur Day (Nintendo 64)
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3) Silver Sonic & Egg Mech (Genesis)
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4) General Razorbeard -- Rayman 2: The Great Escape (Dreamcast/Nintendo 64)
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5) Kaptain K. Rool -- Donkey Kong Country 2: Diddy Kong's Quest (Super Nintendo)
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6) Ganon -- Legend of Zelda: Ocarina of Time (Nintendo 64)
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7) Mother Brain -- Super Metroid (SNES)
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8) Psycho Mantis -- Metal Gear Solid (Playstation)
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9) Marx -- Kirby's Super Star (Super Nintendo)
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10) Dr. Andross -- Starfox 64 (Nintendo 64)
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11) Kefka -- Final Fantasy VI (Super Nintendo)
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12) Magician -- House of the Dead 2 (Dreamcast)
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13) Destroyman -- No More Heroes (Wii)
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14) N. Gin -- Crash Bandicoot 2 (Playstation)
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15) TV -- Clockwork Knight (Sega Saturn)
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16) Adam the Clown -- Dead Rising (X-Box 360)
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17) Akuma -- Street Fighter 2 (Arcade)
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18) Wild Dog -- Time Crisis (Arcade/Playstation)
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19) Aaron -- Clive Barker's The Undying (PC)
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20) Gulpo -- Nights into Dreams (Sega Saturn)
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P.S. I'll add explanations to each ranking and the memorable thing about them as the night goes on.
-- Jordan