update
UPDATE: MY qual is tentatively scheduled for September 13, 2005.
I have been BUSY at work, getting stuff put together so that I can do RNAi knockdowns on my kinases, and then take pictures of them. First things first, I have to get tissue culture up and running.
That's getting there-- I learned a secret from our master chef, so to speak: don't let the cells go to confluence and they'll be healthier-- as in, replate them when they're less than 90% by far.
Ok I can do that. ;)
I also got my cover slips ready today-- steep them in acid, then wash and coat with protein. Funfunfun, but now I have 36 wells hot n ready to go. Tomorrow, I hope to split my cells one last time, then plate and get this show on the road. I figure that by this weekend I could be starting my wound healing assays.
To be analyzed and images taken at the beginning of next week?
We shall see. So much to think about, so little time!
Also, I need to nail down a few things about my project:
*whether the phosphorylation sites can be determined on a toolkit (Expasy?? Biology Workbench?)
*how to do functional analysis of this kinase
*metamorph software and how to use it to quantitate lamellipodium
*How to quantitate migration using metamorph (LILA's papers?)
*brush up on physiological relevance of PI4K.
*get pictures into slides and start laying out powerpoint presentation
*what is the time for the presentation? does it have to be 20-30 min or could it be longer?
*vesicle transport of PIP to PM as alternative in my model
*Make a model slide of what I think is going on--different PI4K at different functions, with possible shuttling of kinases with different protein-protein interactions modulating, and possibly a nuclear shuttling process too.
*Guess from my committee's interests what they will be asking about
*REVIEW GILBERT NOTES FROM SERVICE COURSES!!! HE KNOWS I KNOW THIS!
*ask about imaging questions, raspberry possibly.
Other things:
*don't panic.
*shopping therapy?
--Get a powersuit. Macy's Woman is my playground.
--hair highlights may help me achieve my Ultimate Persona. Plus, I have a coupon. Make an appt.
--Ultimate Persona requires Ultimate Shoes that are paradoxically stylish, but comfortable, and somehow they say "yes I am capable of writing and thinking like a fabulous scientist."
Now?
*sleep
I have been BUSY at work, getting stuff put together so that I can do RNAi knockdowns on my kinases, and then take pictures of them. First things first, I have to get tissue culture up and running.
That's getting there-- I learned a secret from our master chef, so to speak: don't let the cells go to confluence and they'll be healthier-- as in, replate them when they're less than 90% by far.
Ok I can do that. ;)
I also got my cover slips ready today-- steep them in acid, then wash and coat with protein. Funfunfun, but now I have 36 wells hot n ready to go. Tomorrow, I hope to split my cells one last time, then plate and get this show on the road. I figure that by this weekend I could be starting my wound healing assays.
To be analyzed and images taken at the beginning of next week?
We shall see. So much to think about, so little time!
Also, I need to nail down a few things about my project:
*whether the phosphorylation sites can be determined on a toolkit (Expasy?? Biology Workbench?)
*how to do functional analysis of this kinase
*metamorph software and how to use it to quantitate lamellipodium
*How to quantitate migration using metamorph (LILA's papers?)
*brush up on physiological relevance of PI4K.
*get pictures into slides and start laying out powerpoint presentation
*what is the time for the presentation? does it have to be 20-30 min or could it be longer?
*vesicle transport of PIP to PM as alternative in my model
*Make a model slide of what I think is going on--different PI4K at different functions, with possible shuttling of kinases with different protein-protein interactions modulating, and possibly a nuclear shuttling process too.
*Guess from my committee's interests what they will be asking about
*REVIEW GILBERT NOTES FROM SERVICE COURSES!!! HE KNOWS I KNOW THIS!
*ask about imaging questions, raspberry possibly.
Other things:
*don't panic.
*shopping therapy?
--Get a powersuit. Macy's Woman is my playground.
--hair highlights may help me achieve my Ultimate Persona. Plus, I have a coupon. Make an appt.
--Ultimate Persona requires Ultimate Shoes that are paradoxically stylish, but comfortable, and somehow they say "yes I am capable of writing and thinking like a fabulous scientist."
Now?
*sleep