(no subject)
LAB LAB LAB LAB LAB is my life right now. I have not NOT been in the lab since Sunday of LAST week.
Weekend labness, o joy.
PCR is shit right now. I don't know what's worse: the possibility that I'm just doing a shit job of PCR, or the possibility that the cDNA is screwed up. Former possibility, my grad student and potentially everyone in the lab thinks I'm a fucking idiot. Latter possibility, shitloads of work figuring out which cDNA is screwed and making new cDNA + potentially having to go all the way back to RNA extraction to not run out of the cDNA we need for all the genes we have to look at, ahhh
AHHH
Weekend labness, o joy.
PCR is shit right now. I don't know what's worse: the possibility that I'm just doing a shit job of PCR, or the possibility that the cDNA is screwed up. Former possibility, my grad student and potentially everyone in the lab thinks I'm a fucking idiot. Latter possibility, shitloads of work figuring out which cDNA is screwed and making new cDNA + potentially having to go all the way back to RNA extraction to not run out of the cDNA we need for all the genes we have to look at, ahhh
AHHH