And it's come to this...
Title: Time lapse videomicroscopy to determine neurotoxicity to amphetamines in a PC12 cell culture during continuous perfusion
Abstract: The rat pheochromocytoma PC12 cell line has become a commonly employed model system for studies of neuronal development. The conditions for cell culture are well established in the group and initial studies on the neurotoxic effects of amphetamines as determined by cell viability assays have been performed. The main aim of my project is to establish the optimum conditions for time-lapse video-microscopy of this cell line during treatment with drugs. Initially, the optimal growth conditions for the PC12 cell line in the perfusion chamber sited on an inverted microscope will be determined. The effect of drug perfusion on three parameters will be determined (a) calcium release as determined using the fluorescent marker fura-2am, (b) cell viability using the Vybrant kit and (c) the level of apoptosis as determined using flourescent-activated cell sorting. Results of my study will establish the techniques for perfusion studies and cell development in a microincubator.
This is what is going to be consuming every waking moment of my life for the rest of the year. Unfortunately the thesis involving taking rectal temperatures of rodents off their trolly on ecstasy was given to another girl. I was so the man for that job, I'm a pro when it comes to anal insertion. Plus I'm dying to know just how toasty my hoop is...sigh. Well at least with all this flourescent measuring techniques I'll be able to work on my tan in between the tears of boredom that is. I have to arrange my first meeting with the supervisor tomorrow, so gotta be all pepped...."Oh my god, is that jumper cashmere? Gorgeous on you..."
Abstract: The rat pheochromocytoma PC12 cell line has become a commonly employed model system for studies of neuronal development. The conditions for cell culture are well established in the group and initial studies on the neurotoxic effects of amphetamines as determined by cell viability assays have been performed. The main aim of my project is to establish the optimum conditions for time-lapse video-microscopy of this cell line during treatment with drugs. Initially, the optimal growth conditions for the PC12 cell line in the perfusion chamber sited on an inverted microscope will be determined. The effect of drug perfusion on three parameters will be determined (a) calcium release as determined using the fluorescent marker fura-2am, (b) cell viability using the Vybrant kit and (c) the level of apoptosis as determined using flourescent-activated cell sorting. Results of my study will establish the techniques for perfusion studies and cell development in a microincubator.
This is what is going to be consuming every waking moment of my life for the rest of the year. Unfortunately the thesis involving taking rectal temperatures of rodents off their trolly on ecstasy was given to another girl. I was so the man for that job, I'm a pro when it comes to anal insertion. Plus I'm dying to know just how toasty my hoop is...sigh. Well at least with all this flourescent measuring techniques I'll be able to work on my tan in between the tears of boredom that is. I have to arrange my first meeting with the supervisor tomorrow, so gotta be all pepped...."Oh my god, is that jumper cashmere? Gorgeous on you..."