OMG!!! I love the books and I LOVE the site!!
"Molecular Cloning: A Laboratory Manual" written by Sambrook, Fritch and Maniatis was the FIRST book that was recommended to me to use as a reference even before I stepped foot into a molecular biology lab... gosh what a long time ago that was! What a brilliant book! The stuff I learned from there I could never learn anywhere else. To understand what a truly magnificent book it is, and the impact that it has had on many generations of molecular biologists, read this:
http://www.cshblogs.org/cshprotocols/2007/10/22/molecular-cloning-aka-maniatis-aka-the-bible-at-25/ Not surprisingly, Cold Spring Harbor Lab (is there any other place in the world that has had the same kind of aura that CSHL has had in the minds of biologists? For one, there was "Molecular Cloning", the undefeated champ among Mol Bio books, for another there was Charles Janeway, who wrote the undefeated champ among Immunology books, who was ALSO from CHSL... to me, CSHL used to seem like a place of magic- where people literally did extraordinary things. One of the pictures in the Immunology book was Janeway kayaking on a lake, with a pretty little building in the background. The scene was from an autumn and the place seemed completely enchanted, with the sparking waters, the sun setting and the little building peeking out from behind a bunch of trees. The caption said "Charles Janeway at the Cold Spring Harbor"- what an indelible mark that picture made in my head about what the laboratory must be like!
I was so enchanted with my idea of the place that I didn't even apply to SUNY Stony Brook (which has close collaborations with CSHL), just because I thought I would find myself too dumb to be amongst such hallowed people! Well, at least until James Watson did his whole "Some people in the world are cleverer than others" stint on TV. Seeing that and the ensuing scrambling by the CSHL chaps to disassociate themselves from Watson made me realize the place is pretty much the same as every other place. Nevertheless, CSHL books hold a place close to my heart, and CSHL workshops and lectures are still very highly regarded by the rest of the world.)...... shoot, now I've forgotten my original train of thought.
Anyway.... like I said, read that article I've linked above. I stumbled across it when I googling up to see why Ed 3 did not have Maniatis listed as an author- did Sambrook and he have a falling out or something? I realized after doing so that most of the Mol Cloning protocols given in the book are probably already online... and that's what CSH Protocols has, in addition to a bunch of things not found in the book. I'm so excited!