i'm such a fuckin genius
So I'm such a fuckin genius, so i had to stay at work for 11 hrs. It took me a lil while to get started today. I got all my stuff together, labeled my tubes and everything. So I go to put them into the centrifuge for their hr spin and it was difficult putting them in, so i thought it was strange and went to look at what bin i got my tubes out of. It was the wrong bin, I'm so used to getting the hiv ultra tubes out of the top bin and didn't know people used another size. So I had to peel the labels off the old tubes and transfer the samples into the new tubes. So after I did that, it was time to put the tubes in the centrifuge, well yeah it wasn't at 4 degrees bc viral load people come in on tuesday, so it's usually already cold for when i get there. Yeah that put me really behind. When I finally got back into things, I forgot to vortex my tubes after putting in the isopropanol, which means the RNA is prolly fucked. I forgot to put isopropanol in one tube which is prolly fucked. I got done isolating around 4 something and decided i might as well amp it then it could be stopped after that step and will plate it tomorrow. So I left at 7pm, having arrived a few mins after 8am. I imagine I will prolly have a large check bc of it. I also imagine that when I plate and get it read, my results will be all fucked up. Oh well, i'm still on practice runs, at least I learned a lot of what not to do. Hopefully tomorrow things will go better and i'll be smarter.