Blaine Anderson - Mark Cohen
A struggling Jewish documentary filmmaker and the narrator of the show. He is Roger's and Collins's roommate until Collins moves out; he is also Maureen's ex-boyfriend.
Sam Anderson - Roger Davis
A once successful but now struggling musician who is HIV positive and an ex-junkie. He hopes to write one last meaningful song before he dies. He is having a hard time coping with the fact that he, along with many others around him, knowing that they are going to die.
Marley Rose - Mimi Marquez
A club dancer and drug addict. She lives downstairs from Mark and Roger, and is Roger's love interest that, like him, has HIV. She is also Benny's ex-lover.
Jake Puckerman - Tom Collins
A gay anarchist with AIDS. Collins dreams of opening a restaurant in Santa Fe, where the problems in New York will not affect him and his friends.
Unique - Angel Dumott Schunard
A young drag queen, street percussionist with AIDS. He is Collins's love interest.
Tina Cohen-Chang - Maureen Johnson
A lesbian performance artist who is Mark's ex-girlfriend and Joanne's current girlfriend. She is very flirtatious and cheated on Mark a lot.
Brittany S. Pierce - Joanne Jefferson
An Ivy League-educated public interest lawyer and a lesbian. Joanne is the woman for whom Maureen left Mark.
Artie Abrams - Benjamin “Bennie” Coffin III
Landlord of Mark, Roger and Mimi's apartment building and ex-roommate of Mark, Collins, Roger, and Maureen. Now married to Alison Grey of the Westport Greys, a very wealthy family involved in real estate, and he is considered a yuppie scum and a sell-out by his ex-roommates. He is also Mimi's ex-lover, although he considers himself her ex-boyfriend.
Joe Hart - Squeegee Man
A homeless person who chants "Honest living!" over and over.
Sugar Motta - Alexi Darling
The producer of Buzzline who tries to employ Mark after his footage of the riot makes primetime.