Cloning Wierd Science II (Geek Alert)
So in discussion with a fairly knowledgeable gentleman I found a flaw in my original cloning method. During the first stage of meiosis (production of the daughter nuclei) the chromosomes can undergo genetic recombination. This recombination would prevent my original method from gathering a complete genome of the donors DNA.
Instead I have a new plan! The entire genome would be available in the desired demethylated form by simply gathering the two gametes produced from a single daughter nuclei in the second stage of meiosis.
Simply extracting the nuclei from both of these gametes and inserting both of them into a denucleated human egg cell would be sufficient to produce an exact clone of the donor.
The original challenge however is to produce a female clone from a male donor. Although this would be much harder, I believe it could be achieved by these additional steps:
Instead I have a new plan! The entire genome would be available in the desired demethylated form by simply gathering the two gametes produced from a single daughter nuclei in the second stage of meiosis.
Simply extracting the nuclei from both of these gametes and inserting both of them into a denucleated human egg cell would be sufficient to produce an exact clone of the donor.
The original challenge however is to produce a female clone from a male donor. Although this would be much harder, I believe it could be achieved by these additional steps:
- Gather the two gametes from the other daughter cell produced in the first stage of meiosis.
- Use Gel Electrophoresis to separate and extract the X chromosome from this other daughter-cells gametes.
- Use Gel Electrophoresis to separate the Y chromosome from our first daughter-cells gametes.
- Substitute the separated X for the Y.
- Gather up the chromosomes now with two identical X chromosomes and insert into the denucleated human egg cell.
- Find a "comfortable" turkey baster.