what, even?
I was trying to explain to Post-Doc that I have to use the confocal microscope to take slices of the cells I'm imaging because they are mitotic and therefore round like beach-balls, where interphase cells are relatively flat, and that various parts of the cellular structure wouldn't be in focus if I simply focused on one plane.
She suggested that I "squish the coverslip to the slide" to flatten it and get everything in one plane. And she was completely sincere about this being good advice.
I was speechless. Seriously. Squish them flat. Because that'll maintain all the 3D structure I'm trying to investigate. Really. How did she earn a Ph.D.? HOW?
Crossposted at http://asimplechord.dreamwidth.org/534575.html; comment as/where you wish.
She suggested that I "squish the coverslip to the slide" to flatten it and get everything in one plane. And she was completely sincere about this being good advice.
I was speechless. Seriously. Squish them flat. Because that'll maintain all the 3D structure I'm trying to investigate. Really. How did she earn a Ph.D.? HOW?
Crossposted at http://asimplechord.dreamwidth.org/534575.html; comment as/where you wish.