Holy Mother, FINALLY
Last night my dream involved making out with Sandro Nesta. Dear god, thank you brain. Well, except for the part where I felt guilty about it.
So there was a bunch of... something happening, which I don't really remember, but the end result was I had to go to this place to do this thing. But to get there, I had to go through this maze kind of thing, which would ask me questions and then give me doors with possible answers on them, and I'd have to go through the door with the correct answer on it or I wouldn't be able to make it through the maze, and also I'd die horribly. (Look, it would be a dream of mine if there wasn't death and horror involved somehow.) There were like giant spiders behind the wrong doors or something. Maybe giant zombie spiders.
The thing was, the "questions" were all about the proper way to isolate RNA for a Northern blot. Like, the first question was "What do you initially suspend the cells in", and the doors were labeled:
1) Phenol-chloroform
2) Tris-HCL
3) Trizol
And I had to go through the door labeled "Trizol". And yeah, it went through the whole protocol. I can never escape work, it looks like.
Anyway, I got to the last question, which was something like, "at what temperature do you heat your RNA pellet to facilitate resuspension", and it was in this big lecture hall like one of the ones from uni, and there was Sandro, sitting in the front row. And when I walked in, he turned around and said, "Oh, I'm so glad you're here, I really don't know anything about RNA". And I'm thinking, "Why the hell is Alessandro Nesta in a maze involving the correct way to isolate RNA?" But I wasn't going to, er, look a gift horse in the mouth, so I took him through the correct door (that's "55-65°C", if you're curious), and it led outside, to some very beautiful countryside by the sea. Looked a bit like south Wales, actually.
And instead of going and doing whatever it was I went through the maze for, Sandro led me to a big caffeteria, filled with Milan players, and I was very awkwardly "erm, I don't quite belong here, can I leave now?" But Sandro went and got me food and sat down next to me and then put his arms around me, and on the one hand I was thinking, "er, didn't we just meet, like ten minutes ago, aren't you moving a bit fast", and on the other I was thinking, "why are you putting your arms around me WHERE THE HELL IS GABRIELLA", but I was also squeeing a bit inside.
And so I ate lunch and cuddled up to Sandro, and afterwards Paolo (we were sitting at his table, and he seemed completely unfazed that Sandro had brought in some strange redhead and was cuddling with her at the lunch table, but then he's Paolo and would probably be unfazed by everything up to and including giant zombie spiders) said, "well, we're going to go make a 3-D human statue in the ocean now" (I kid you not). And I was very, "erm, ok, I think I'll just go then", but Sandro kept insisting I come. "It's very beautiful," he said. "It's this giant sculpture composed of all of our bodies, and it's quite emotionally moving until we all get knocked down by the waves." And then he told me he had actually been waiting in the RNA maze for me specifically.
So I went along, and I was in the backseat of the car with Sandro (Paolo was driving and Gila was in the front seat), and the landscape was really starting to look like south Wales, and Paolo was describing this new human-statue-in-the-ocean artform Milan was doing, and then all of a sudden Sandro was kissing me.
And it was quite nice, really, except then I thought WHERE THE HELL IS GABRIELLA I SHOULD NOT BE DOING THIS, and woke up.
*sigh* It seems I can't escape guilt (and zombie spiders) even in my dreams.
So there was a bunch of... something happening, which I don't really remember, but the end result was I had to go to this place to do this thing. But to get there, I had to go through this maze kind of thing, which would ask me questions and then give me doors with possible answers on them, and I'd have to go through the door with the correct answer on it or I wouldn't be able to make it through the maze, and also I'd die horribly. (Look, it would be a dream of mine if there wasn't death and horror involved somehow.) There were like giant spiders behind the wrong doors or something. Maybe giant zombie spiders.
The thing was, the "questions" were all about the proper way to isolate RNA for a Northern blot. Like, the first question was "What do you initially suspend the cells in", and the doors were labeled:
1) Phenol-chloroform
2) Tris-HCL
3) Trizol
And I had to go through the door labeled "Trizol". And yeah, it went through the whole protocol. I can never escape work, it looks like.
Anyway, I got to the last question, which was something like, "at what temperature do you heat your RNA pellet to facilitate resuspension", and it was in this big lecture hall like one of the ones from uni, and there was Sandro, sitting in the front row. And when I walked in, he turned around and said, "Oh, I'm so glad you're here, I really don't know anything about RNA". And I'm thinking, "Why the hell is Alessandro Nesta in a maze involving the correct way to isolate RNA?" But I wasn't going to, er, look a gift horse in the mouth, so I took him through the correct door (that's "55-65°C", if you're curious), and it led outside, to some very beautiful countryside by the sea. Looked a bit like south Wales, actually.
And instead of going and doing whatever it was I went through the maze for, Sandro led me to a big caffeteria, filled with Milan players, and I was very awkwardly "erm, I don't quite belong here, can I leave now?" But Sandro went and got me food and sat down next to me and then put his arms around me, and on the one hand I was thinking, "er, didn't we just meet, like ten minutes ago, aren't you moving a bit fast", and on the other I was thinking, "why are you putting your arms around me WHERE THE HELL IS GABRIELLA", but I was also squeeing a bit inside.
And so I ate lunch and cuddled up to Sandro, and afterwards Paolo (we were sitting at his table, and he seemed completely unfazed that Sandro had brought in some strange redhead and was cuddling with her at the lunch table, but then he's Paolo and would probably be unfazed by everything up to and including giant zombie spiders) said, "well, we're going to go make a 3-D human statue in the ocean now" (I kid you not). And I was very, "erm, ok, I think I'll just go then", but Sandro kept insisting I come. "It's very beautiful," he said. "It's this giant sculpture composed of all of our bodies, and it's quite emotionally moving until we all get knocked down by the waves." And then he told me he had actually been waiting in the RNA maze for me specifically.
So I went along, and I was in the backseat of the car with Sandro (Paolo was driving and Gila was in the front seat), and the landscape was really starting to look like south Wales, and Paolo was describing this new human-statue-in-the-ocean artform Milan was doing, and then all of a sudden Sandro was kissing me.
And it was quite nice, really, except then I thought WHERE THE HELL IS GABRIELLA I SHOULD NOT BE DOING THIS, and woke up.
*sigh* It seems I can't escape guilt (and zombie spiders) even in my dreams.