waiting to digest
Yup, that's right. I have about 12 more minutes until the digestion if finished. No, i'm not talking about my lunch, but rather a piece of plasmid DNA that i fondly call pNH1. (p = plasmid, NH = my initials, 1 = the first one i have made) Yes, i have a little piece of DNA to call my own which is presently sitting in a 37 degree C incubator being knawed on by an enzyme called BamH1. It's a restriction enzyme digest of plasmid DNA. This is fancy science talk for something that's not all that exciting. I'm taking a circular piece of DNA and making it into a straight piece of DNA. After that i get to make a radioactive piece of RNA based off of it. That should be pretty cool.
Besides just sitting on my ass waiting for this to happen, i've been thinking. I'm really really really nervous about my friday at 4. Friday May 13th at 4:00 pm is my solo classical guitar concert. Eeeeeeeeeeek!!!!! So far there are a lot of people whose opinions i care about who will be in the audience. I haven't learned the courant or Milonga yet. I have a little over 2 weeks. I'm terrified. I know i can play...but under that kinda pressure.... I guess i'll just have to wait and see what happens. Work really hard and hope for the best. But i just can't stop thinking about it and imagining disaster. grrrrr..... Years of work has led up to that precise moment. I'm not going to give up. It's just too damn important. That's the problem. I guess only time will tell.
Side note: Last Tuesday at around 11 there was a shooting in my yard. Some namesless goon (i don't know what he did) was running from the cops. One fired at the other. A few shots. I was in bed drifting off on a cloud of sedatives. Very odd. I got up briefly to see the front yard full of police cars, dogs, and policemen milling around. Whoo hoo. I now officially live in the ghetto.
Besides just sitting on my ass waiting for this to happen, i've been thinking. I'm really really really nervous about my friday at 4. Friday May 13th at 4:00 pm is my solo classical guitar concert. Eeeeeeeeeeek!!!!! So far there are a lot of people whose opinions i care about who will be in the audience. I haven't learned the courant or Milonga yet. I have a little over 2 weeks. I'm terrified. I know i can play...but under that kinda pressure.... I guess i'll just have to wait and see what happens. Work really hard and hope for the best. But i just can't stop thinking about it and imagining disaster. grrrrr..... Years of work has led up to that precise moment. I'm not going to give up. It's just too damn important. That's the problem. I guess only time will tell.
Side note: Last Tuesday at around 11 there was a shooting in my yard. Some namesless goon (i don't know what he did) was running from the cops. One fired at the other. A few shots. I was in bed drifting off on a cloud of sedatives. Very odd. I got up briefly to see the front yard full of police cars, dogs, and policemen milling around. Whoo hoo. I now officially live in the ghetto.